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Average 96 stars, based on 1 article reviews
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BMDMs pretreated with Aquamin (2mg/ml) for 3 h prior to stimulation with the corresponding TLR agonists overnight, LPS (100ng/ml) for TLR4 stimulation, Poly(I:C) (100ug/ml) for TLR3 stimulation and CpG (3μM) for TLR9 stimulation. (a)IL-6 and (b) TNF-alpha cytokine levels in the cell culture supernatants by ELISA. Data are expressed as mean ±sem from 3 independent experiments. **p<0.01, ***p<0.001 vs LPS alone using one way ANOVA statistical test .

Journal: bioRxiv

Article Title: Aquamin a marine derived multi mineral attenuates toll like receptor mediated inflammatory responses in macrophages

doi: 10.64898/2026.01.19.700098

Figure Lengend Snippet: BMDMs pretreated with Aquamin (2mg/ml) for 3 h prior to stimulation with the corresponding TLR agonists overnight, LPS (100ng/ml) for TLR4 stimulation, Poly(I:C) (100ug/ml) for TLR3 stimulation and CpG (3μM) for TLR9 stimulation. (a)IL-6 and (b) TNF-alpha cytokine levels in the cell culture supernatants by ELISA. Data are expressed as mean ±sem from 3 independent experiments. **p<0.01, ***p<0.001 vs LPS alone using one way ANOVA statistical test .

Article Snippet: The following toll-like receptor (TLR) agonists were used TLR3 Poly(I:C) HMW (100ug/ml), TLR 9 CpG ODN 1826 (3μM) (both InvivoGen) and TLR4 LPS (100ng/ml) from Escherichia coli O55:B5 (Sigma Aldrich, L6529). hPBMCs or mBMDMs were first treated with Aqaumin (0.5,1,2 mg/ml) for 3h prior to TLR agonist stimulation for a further 4-12h.

Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay